<p>In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by SDS-PAGE, western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE-binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunit, both displaying micro-heterogeneity, and epitope mapping of Ana o 3 has been performed.<br></p>